yknX [2018-10-11 18:49:50]

ABC-type antimicrobial peptide transporter (permease) for the export of the [protein|820635420B3980B620F3E3D7ACF7EDE9DC6275AD|SdpC] toxin

locus: BSU14350

pI: 5.18

mw: 41.55 kDa

protein length: 377 aa

gene length: 1131 bp

function: resistence against [protein|820635420B3980B620F3E3D7ACF7EDE9DC6275AD|SdpC] toxin

product: ABC-type antimicrobial peptide exporter (permease)

essential: no

synonyms

Genomic Context

categories

• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.13|Resistance against toxins/ antibiotics]
• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.2|Cell envelope stress proteins (controlled by SigM, V, W, X, Y)]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 1,504,282 → 1,505,415

The protein

Structure

• [PDB|4DK0] (from Actinobacillus actinomycetemcomitans, 24% identity)

[SW|Localization]

• membrane protein with a large extracytoplasmic domain [Pubmed|22707703]

Expression and Regulation

Operons

• genes: [gene|70D7098BEBD5E53B4B9B8C3394FAAF972C65EE22|yknU]-[gene|C6B0ACB9D9703DD8141FD7D9D62DB22FB48F458B|yknV]-[gene|916419C4ED113778488EBC558A35810498028B02|yknW]-[gene|FD270195901B89DAAAD134DB9DE729670A9BDECF|yknX]-[gene|FE3A01BEA974C5E32C68DC600C800E5EF101B34F|yknY]-[gene|9680326BAA94A503E2A5C6F0A2479AE0B0467B4C|yknZ]
  description: [Pubmed|22383849]

• genes: [gene|916419C4ED113778488EBC558A35810498028B02|yknW]-[gene|FD270195901B89DAAAD134DB9DE729670A9BDECF|yknX]-[gene|FE3A01BEA974C5E32C68DC600C800E5EF101B34F|yknY]-[gene|9680326BAA94A503E2A5C6F0A2479AE0B0467B4C|yknZ]
  description: [Pubmed|9987136]

  sigma factors

  • [protein|3D974DD2967C7F8FD4E3C2AF42617B8AE9F0296D|SigW]: sigma factor, [Pubmed|9987136], in [regulon|3D974DD2967C7F8FD4E3C2AF42617B8AE9F0296D|SigW regulon]

  regulatory mechanism

  • [protein|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB]: repression, [Pubmed|12076816], in [regulon|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB regulon]

  regulation

  • repressed during logrithmic growth ([protein|search|AbrB]) [Pubmed|12076816]

Biological materials

Mutant

• MGNA-B349 (yknX::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1348 NBRP B. subtilis, Japan]
• BKE14350 (Δ[gene|FD270195901B89DAAAD134DB9DE729670A9BDECF|yknX]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE14350 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TCCGATCCAGACTTTTTTCA, downstream forward: _UP4_GACGGAATGGAAGTGAAATC
• BKK14350 (Δ[gene|FD270195901B89DAAAD134DB9DE729670A9BDECF|yknX]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK14350 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TCCGATCCAGACTTTTTTCA, downstream forward: _UP4_GACGGAATGGAAGTGAAATC

References

• 16629676,9987136,12076816